COLLAGEN SYNTHESIS AND ASSEMBLY
Collagen synthesis and assembly follows the normal pathway for a secreted protein. The activation of fibroblasts in the connective tissues results in an increase in the production of collagen. The mechanism of collagen formation is well known.
The genetic information encoded in the DNA is “read” (transcription process in the nucleus), the mature mRNA is then transported to the cytoplasm and “translated” (translation process) to produce the single polypeptide chains (α-chains) at the rough endoplasmatic reticulum (ER). Each α-chain has a terminal peptide sequence, also known as trimerization domain, which drives the assembly of the longer precursors, which are termed procollagens.
Ribosome-bound mRNA is translated into growing preprocollagen molecules that are assembled inside the fibroblasts, into the lumen of the ER.